Editor: Robert F. Klie Triclosan Resistance in Francisella Tularensis: a Site-directed Mutagenesis Study of the Fabi Enzyme

نویسنده

  • Robert F. Klie
چکیده

On the Cover (from left to right): 1. Illustration of Concentrated Solar Power plant layout consisting of a solar field, thermal electric storgae, and power block subcomponents. (see E. Flores, page 28); 2. A92V ftFabI with residue 92 as valine. (see N. Silas et al., page 4); 3. Photo of traditional electrical meters. (see A. Gilbert, page 22). A refereed journal for undergraduate research in the pure & applied sciences, mathematics and engineering. Introduction Dear Colleagues, Welcome to the eighth edition of the Journal of Undergraduate Research at the University of Illinois at Chicago, largely devoted to papers for the LAS 493 course on Energy Storage for the Grid and Transportation in Fall 2014. The class consisted of mostly graduate students, with a few advanced undergraduates as well, surveying the motivations, challenges and opportunities for storing energy for the electricity grid and electric cars. The course focused on next generation uses of electricity storage, going well beyond the personal electronics, such as cell phones, music players, camcorders, laptops and tablets that dominate battery use today. Currently, transportation and the grid account for 67% of all energy consumed in the United States, compared to only 2% for personal electronics. In energy terms, a fraction of the energy used in the grid and transportation represents an order of magnitude increase over the present market for personal electronics. The batteries needed for the grid and transportation are likely to be qualitatively different from the lithium-ion batteries now in widespread use. Although lithium-ion batteries increase in performance and decrease in cost by 5%-10% per year, they cannot yield the transformational advances in cost and performance needed for widespread deployment of electric cars and of electricity storage on the grid. The papers in this volume review and explore various topics and technologies that could emerge to meet these needs, and the game-changing impact these technologies may have on the way we think about transportation and electricity. Many of the papers were inspired by the lectures presented in the class, given by electricity storage experts drawn from the University of Illinois at Chicago, the Joint Center for Energy Storage Research (JCESR), the battery research programs at Argonne National Laboratory, Illinois Institute of Technology and other universities and companies, including General Motors. The lectures covered beyond-lithium-ion battery technology, electrochemical capacitors, solar and wind generation, electric cars of the future, long-distance electricity transmission, distributed energy in microgrids, and …

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Structure of the Francisella tularensis enoyl-acyl carrier protein reductase (FabI) in complex with NAD(+) and triclosan.

Enoyl-acyl carrier protein reductase (FabI) catalyzes the last rate-limiting step in the elongation cycle of the fatty-acid biosynthesis pathway and has been validated as a potential antimicrobial drug target in Francisella tularensis. The development of new antibiotic therapies is important both to combat potential drug-resistant bioweapons and to address the broader societal problem of increa...

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3-substituted indole inhibitors against Francisella tularensis FabI identified by structure-based virtual screening.

In this study, we describe novel inhibitors against Francisella tularensis SchuS4 FabI identified from structure-based in silico screening with integrated molecular dynamics simulations to account for induced fit of a flexible loop crucial for inhibitor binding. Two 3-substituted indoles, 54 and 57, preferentially bound the NAD(+) form of the enzyme and inhibited growth of F. tularensis SchuS4 ...

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Site-directed Mutagenesis in Francisella Tularensis by Allelic Replacement

Francisella tularensis is a Gram-negative, facultative intracellular coccobacillus and the etiologic agent of tularemia for a wide variety of vertebrate and invertebrate animal species. Several species and subspecies of Francisella are currently recognized. However, the majority of infections are caused by F. tularensis subspecies tularensis (type A) and subspecies holarctica (type B). Given th...

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The Francisella tularensis FabI enoyl-acyl carrier protein reductase gene is essential to bacterial viability and is expressed during infection.

Francisella tularensis is classified as a category A priority pathogen and causes fatal disseminated disease in humans upon inhalation of less than 50 bacteria. Although drugs are available for treatment, they are not ideal because of toxicity and route of delivery, and in some cases patients relapse upon withdrawal. We have an ongoing program to develop novel FAS-II FabI enoyl-ACP reductase en...

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Construction of targeted insertion mutations in Francisella tularensis subsp. novicida.

Francisella tularensis is one of the most deadly bacterial agents, yet most of the genetic determinants of pathogenesis are still unknown. We have developed an efficient targeted mutagenesis strategy in the model organism F. tularensis subsp. novicida by utilizing universal priming of optimized antibiotic resistance cassettes and splicing by overlap extension (SOE). This process enables fast an...

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تاریخ انتشار 2015